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Paternity Testing

Paternity testing uses DNA analysis to ascertain whether or not a man is the father of a particular child. A DNA sample can simply be collected by swabbing the inside of the cheek of the mouth to obtain a cheek cell sample of both the child and man, since DNA is found in all the cells of the body. The Polymerase Chain Reaction (PCR) test is used to analyze the DNA sample in the lab. The DNA in the test tube is separated from the sample by removing all the other components of the cell including proteins. The PCR process utilizes DNA polymerase to replicate copies of specific DNA strands. There are 3 steps in the PCR process:

1. Separation of the DNA strands is done by heating the material to 90 -96 0C in order to denature the specific genetic material into two double helix DNA chains. When the two strands separate, each strand is used as a template when a copy is made by DNA polymerase.

2. At 55 0C, a short sequence of nucleotides attaches to the ends of the DNA strands.

3. The temperature of the test tube is increased to 75 0C, and a complete copy of each DNA strand is made (replication).

The specific target loci of the father and child DNA sample is examined and compared. Two numbers are generated one will match the biological mother’s numbers and the other the biological father’s numbers.

Probability of paternity value of 99.99% means that the man tested is the biological father of the child.

Probability of paternity value of 0% means that the man tested is not the biological father of the child.

-nandi and marissa



super fact sunday

  • Humans and cabbage has about 40-50 % similar DNA

shocked patrick

  • If you uncoil your DNA, it would be long enough to reach to the moon and back 6000 times!
  • Boys…here’s a fun fact for you. A single sperm has 37.5MB of DNA information in it. When it is ejaculated this represents a transfer of data around 1,587GB in 3 seconds…WELL! That’s fast…


  • It would take someone to type 60 words per minute and 8 hours a day anddd 50 years to type the human genome..daymmmm

banana dna

dna ugly

Double Strand of the awesomeness!


wordle showing the relevant terms for nucleotides and nucleic acids




 Wikipedia. “Parental testing.” 2014. (accessed 6 Apr 2014). “How DNA Fingerprinting Works.” 2014. (accessed 6 Apr 2014). “Paternity Testing.” 2014. (accessed 6 Apr 2014).

Holy Kaw!. “17 interesting facts about DNA.” 2010. (accessed 6 Apr 2014). “And you thought 4G was fast….” 2014. (accessed 6 Apr 2014)

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