Know your Glycolytic enzymes
We all know that in glycolysis there are 10 reactions controlled by 10 enzymes. These are:
- Hexokinase: It catalyses alpha D-Glucose and ATP to Glucose-6-Phosphate (G6P) and ADP by phosphorylation.
- Phosphoglucose isomerase: This catalyses G6P to its isomer Fructose-6-phosphate (F6P). This occurs by changing the carbonyl oxygen from the C1 position on the G6P to the C2 position on F6P
- Phosphofructokinase: Catalyses F6P and ATP to Fructose-1, 6-Bisphosphate (F1, 6PP) and ADP.
- Fructose-Bisphosphate aldolase: Catalyses F1,6PP to split into Dihydroxyacetone Phosphate (DHAP) and Glyceraldehyde-3-Phosphate (G3P)
- Triosephosphate isomerase: This catalyses DHAP to turn into G3P because only G3P can be used in the reaction.
- Glyceraldehyde-3-phosphate dehydrogenase: Catalyses G3P, NAD and Pi to 1,3 Bisphosphoglycerate (1,3 BPG), NADH and H+
- Phosphoglycerate kinase: Catalyses 1,3 BPG and ADP to 3-Phosphoglycerate and ATP
- Phosphoglycerate mutase: Catalyses 3PG to 2-Phosphoglycerate (2PG)
- Enolase: Catalyses 2PG to Phosphoenolpyruvate (PEP) and H2O.
- Pyruvate kinase: It catalyses PEP and ADP to Pyruvate and ATP.