FOREFATHERS OF ENZYMES
Louis Pasteur was a researcher, biochemist, microbiologist and scientist among other things who engaged in the study of fermentation in 1985 because it was observed that large volumes of beet juice became sour instead of alcoholic as intended. He observed that yeast was instrumental in converting sugar to ethanol and declared that only live yeast could cause this reaction.
Edward Buchner, a Nobel Laureate, disagreed with Pasteur’s statement and proved through an experiment that he could produce an extract of yeast that was free of yeast cells that could still cause fermentation.
James Sumner, an American chemist, experimented with the enzyme urease which he isolated and crystallised. Through his experiments he was the first to prove that urease was a protein, which gave rise to the hypothesis that enzymes were proteins. He achieved this after several failed attempts.
John Burdon Sanderson Haldane, a naturalised Indian scientist, finally hypothesised that weak interactions bind enzymes to substrates. This hypothesis is still accepted to this day. WAY TO GO JOHN!
YouTube. “Enzymes 1 JM podcast.mov.” 2014. http://www.youtube.com/watch?v=qcA57r2gBL8&feature=youtu.be (accessed 5 Mar 2014).
YouTube. “Enzymes JM Part 2.mov.” 2014. http://www.youtube.com/watch?v=FPKAJlgMCbE&feature=youtu.be (accessed 5 Mar 2014).